opuCC [2017-11-24 13:57:55]

glycine betaine/carnitine/choline ABC transporter (binding protein)

locus: BSU33810

pI: 5.69

mw: 33.80 kDa

protein length: 303 aa

gene length: 909 bp

function: compatible solute transport

product: glycine betaine/carnitine/choline ABC transporter (binding protein)

essential: no

synonyms: yvbC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,468,253 → 3,469,164

The protein

Paralogous protein(s)

• [protein|C48798FE13B1521236F5BC5786B9A02D7BC9A336|OpuBC]

Structure

• [PDB|3PPN] [Pubmed|21366542]

[SW|Localization]

• associated to the membrane (via [protein|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|OpuCB]-[protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|OpuCD]) [Pubmed|10092453]
• lipoprotein [Pubmed|10216873]

Expression and Regulation

Operons

• genes: [gene|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA]-[gene|83C45215AC86FACB08CF36EB5F9E6B076D7D0F0F|opuCB]-[gene|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]-[gene|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|opuCD]
  description: [Pubmed|10216873]

  regulatory mechanism

  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

  regulation

  • induced by salt stress [Pubmed|23960087,10216873]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• BKE33810 (Δ[gene|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATTTTCAACAGTGCCA, downstream forward: _UP4_GACTAAGAAAAGAGGTGGAT
• BKK33810 (Δ[gene|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATTTTCAACAGTGCCA, downstream forward: _UP4_GACTAAGAAAAGAGGTGGAT

References

Reviews

• 27935846

Original publications

• 10092453,9925583,10216873,21296969,21366542,23960087,23646920,28256787